Single nuclei RNA-seq of mouse placental labyrinth development

eLife

Robert Blelloch¹  Bryan Marsh² 

[1] Department of Urology, University of California, San Francisco, San Francisco, United States;The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, United States;

关键词: placenta;    mouse;    single nuclei RNA sequencing;    placental development;    labyrinth;    syncytiotrophoblasts;   

DOI  :  10.7554/eLife.60266

来源: DOAJ

【 摘 要 】

The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study.

【 授权许可】

Unknown