Bioactive Phytochemicals of Citrus reticulata Seeds—An Example of Waste Product Rich in Healthy Skin Promoting Agents

Antioxidants

Tarfah Al-Warhi¹  Samy Selim²  Mohammad M. Al-Sanea³  Abeer H. Elmaidomy⁴  Usama Ramadan Abdelmohsen⁵  Ahmed M. Sayed⁶  Sabouni Ibrahim⁷  Mha Albqmi⁷  Ehab M. Mostafa⁸  Mohammed M. Ghoneim⁹ 

[1] Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi Arabia;Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia;Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt;Olive Research Center, Jouf University, Sakaka 72341, Saudi Arabia;Pharmacognosy Department, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia;Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt;

关键词: orange;    Citrus;    hyaluronidase;    xanthine oxidase;    tyrosinase;    docking;   

DOI  :  10.3390/antiox11050984

来源: DOAJ

【 摘 要 】

Phytochemical investigation of Egyptian mandarin orange (Citrus reticulata Blanco, F. Rutaceae) seeds afforded thirteen known compounds, 1–13. The structures of isolated compounds were assigned using 1D and 2D NMR and HRESIMS analyses. To characterize the pharmacological activity of these compounds, several integrated virtual screening-based and molecular dynamics simulation-based experiments were applied. As a result, compounds 2, 3 and 5 were putatively identified as hyaluronidase, xanthine oxidase and tyrosinase inhibitors. The subsequent in vitro testing was done to validate the in silico-based experiments to highlight the potential of these flavonoids as promising hyaluronidase, xanthine oxidase and tyrosinase inhibitors with IC₅₀ values ranging from 6.39 ± 0.36 to 73.7 ± 2.33 µM. The present study shed light on the potential of Egyptian mandarin orange’s waste product (i.e., its seeds) as a skin health-promoting natural agent. Additionally, it revealed the applicability of integrated inverse docking-based virtual screening and MDS-based experiments in efficiently predicting the biological potential of natural products.

【 授权许可】

Unknown