Impact of Cross-Linking of Collagen Matrices on Tissue Regeneration in a Rabbit Calvarial Bone Defect

Materials

Hiroki Katagiri¹  Elena Andrejova¹  Masako Fujioka-Kobayashi¹  Nikola Saulacic¹  Benoit Schaller¹  Niklaus P. Lang¹  Anton Sculean²  Jean-Claude Imber² 

[1] Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, University of Bern, 3012 Bern, Switzerland;Department of Periodontology, University of Bern, 3012 Bern, Switzerland;

关键词: collagen;    cross-linking;    tissue augmentation;    bone formation;   

DOI  :  10.3390/ma14133740

来源: DOAJ

【 摘 要 】

The cross-linking of collagen matrices (Cl_CM) may provide volume-stable enhanced defect regeneration when compared to non-cross-linked matrices (Ncl_CM). The aim of the present study was to investigate the bone forming potential of collagen matrices (CMs) and the effects of cross-linking CMs in a rabbit calvaria defect model. (1) Empty controls (n = 6), (2) Ncl_CM (n = 8), and (3) Cl_CM (n = 8) were selected to be observed for the healing in 10 mm critical-sized calvarial bone defects. The potential for the bone as well as the connective tissue formation were evaluated by micro-CT and histomorphometry at three months post-surgery. There were no statistically significant differences in terms of new bone volume in the defects between the groups. However, the Cl_CM induced significantly greater fibrous tissue regeneration (5.29 ± 1.57 mm²) when compared to the controls (3.51 ± 0.93 mm²) by histomorphometry. The remnants of collagen fibers with immune cells, including macrophages and giant cells, were occasionally observed in the Cl_CM group but not in the Ncl_CM group. In conclusion, the cross-linking of collagen did not influence the potential for bone formation. Nevertheless, Cl_CM might be advantageous for the maintenance of fibrous tissue volume without disturbing bone formation in the defects.

【 授权许可】

Unknown