G2019S LRRK2 mutation facilitates α-synuclein neuropathology in aged mice

Neurobiology of Disease

Nathalie Dutheil¹  Erwan Bezard¹  Ludovico Arcuri²  Salvatore Novello²  Michele Morari³  Sandra Dovero⁴  Derya R. Shimshek⁴ 

[1] CNRS, Institut des Maladies Neurodégénératives, UMR 5293, F-33000 Bordeaux, France;Department of Medical Sciences, Section of Pharmacology, University of Ferrara, National Institute of Neuroscience, Ferrara, Italy;Department of Neuroscience, Novartis Institutes for BioMedical Research, Novartis Pharma AG, 4002 Basel, Switzerland;Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, F-33000 Bordeaux, France;

关键词: α-synuclein;    G2019S knock-in mice;    iba-1;    LRRK2;    Microglia;    Parkinson's disease;   

DOI  :  

来源: DOAJ

【 摘 要 】

Fibrillization of α-synuclein is instrumental for the development of Parkinson's disease (PD), thus modulating this process can have profound impact on disease initiation/progression. Here, the impact of the p.G2019S mutation of leucine-rich repeat kinase 2 (LRRK2), which is most frequently associated with familial and sporadic PD, on α-synuclein pathology was investigated. G2019S knock-in mice and wild-type controls were injected with a recombinant adeno-associated viral vector serotype 2/9 (AAV2/9) overexpressing human mutant p.A53T α-synuclein (AAV2/9-hα-syn). Control animals were injected with AAV2/9 carrying green fluorescent protein. Motor behavior, transgene expression, α-syn and pSer129 α-syn load, number of nigral dopamine neurons and density of striatal dopaminergic terminals were evaluated. To investigate the effect of aging, experiments were performed in 3- and 12-month-old mice, evaluated 20 and 12 weeks after virus injection, respectively. hα-syn overexpression induced progressive motor deficits, loss of nigral dopaminergic neurons and striatal terminals, and appearance of proteinase K-resistant aggregates of pSer129 α-syn in both young and old mice. Although no genotype difference was observed in 3-month-old mice, degeneration of nigral dopaminergic neurons was higher in 12-month-old G2019S knock-in mice compared with age-matched wild-type controls (−55% vs −39%, respectively). Consistently, a two-fold higher load of pSer129 α-syn aggregates was found in 12-month-old G2019S knock-in mice. We conclude that G2019S LRRK2 facilitates α-synucleinopathy and degeneration of nigral dopaminergic neurons, and that aging is a major determinant of this effect.

【 授权许可】

Unknown