Variations in Circulating Active MMP-9 Levels During Renal Replacement Therapy

Biomolecules

Julián Segura¹  Elena Rodríguez-Sánchez¹  Luis Miguel Ruilope¹  Gema Ruiz-Hurtado¹  Jennifer Aceves-Ripoll¹  Judith Abarca-Zabalía¹  Andrea Susmozas-Sánchez¹  José Alberto Navarro-García¹  Manuel Praga²  Evangelina Mérida-Herrero²  Eduardo Hernández²  Teresa Bada-Bosch²  Amado Andrés²  Mario Fernández-Ruiz³  José María Aguado³ 

[1] Cardiorenal Translational Laboratory, Institute of Research i+12 (imas12), Hospital Universitario 12 de Octubre, 28041, Madrid, Spain;Service of Nephrology, Hospital Universitario 12 de Octubre, 28041, Madrid, Spain;Unit of Infectious Diseases, Hospital Universitario 12 de Octubre, 28041, Madrid, Spain;

关键词: matrix metalloproteinase-9;    dialysis;    on-line hemodiafiltration;    high-flux dialysis;    renal replacement therapy;    kidney transplantation;   

DOI  :  10.3390/biom10040505

来源: DOAJ

【 摘 要 】

Renal replacement therapy (RRT) is complicated by a chronic state of inflammation and a high mortality risk. However, different RRT modalities can have a selective impact on markers of inflammation and oxidative stress. We evaluated the levels of active matrix metalloproteinase (MMP)-9 in patients undergoing two types of dialysis (high-flux dialysis (HFD) and on-line hemodiafiltration (OL-HDF)) and in kidney transplantation (KT) recipients. Active MMP-9 was measured by zymography and ELISA before (pre-) and after (post-) one dialysis session, and at baseline and follow-up (7 and 14 days, and 1, 3, 6, and 12 months) after KT. Active MMP-9 decreased post-dialysis only in HFD patients, while the levels in OL-HDF patients were already lower before dialysis. Active MMP-9 increased at 7 and 14 days post-KT and was restored to baseline levels three months post-KT, coinciding with an improvement in renal function and plasma creatinine. Active MMP-9 correlated with pulse pressure as an indicator of arterial stiffness both in dialysis patients and KT recipients. In conclusion, active MMP-9 is better controlled in OL-HDF than in HFD and is restored to baseline levels along with stabilization of renal parameters after KT. Active MMP-9 might act as a biomarker of arterial stiffness in RRT.

【 授权许可】

Unknown