Isolation of Listeria ivanovii from Bulk-Tank Milk of Sheep and Goat Farms—From Clinical Work to Bioinformatics Studies: Prevalence, Association with Milk Quality, Antibiotic Susceptibility, Predictors, Whole Genome Sequence and Phylogenetic Relationships

Biology

Mariangela Caroprese¹  Panagiota I. Kontou²  Natalia G. C. Vasileiou³  Dimitra V. Liagka³  Maria V. Bourganou⁴  Angeliki I. Katsafadou⁴  Dimitris C. Chatzopoulos⁴  Anargyros Skoulakis⁵  Katerina Tsilipounidaki⁶  Zoe Florou⁶  Efthymia Petinaki⁶  Konstantina S. Dimoveli⁷  Charalambia K. Michael⁷  Vasia S. Mavrogianni⁷  George C. Fthenakis⁷  Vasileios G. Papatsiros⁷  Daphne T. Lianou⁷  Eleni I. Katsarou⁷  Peter J. Cripps⁷  Nikolaos Solomakos⁷ 

[1] Department of Agriculture, Food, Natural Resources and Engineering (DAFNE), University of Foggia, 71122 Foggia, Italy;Department of Mathematics, University of Thessaly, 35131 Lamia, Greece;Faculty of Animal Science, University of Thessaly, 41110 Larissa, Greece;Faculty of Public and One Health, University of Thessaly, 43100 Karditsa, Greece;Institute for Bioinnovation, BSRC Alexander Fleming, 16672 Vari, Greece;University Hospital of Larissa, 41110 Larissa, Greece;Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece;

关键词: goat;    Greece;    Listeria;    milk;    phylogenetic analysis;    sheep;   

DOI  :  10.3390/biology11060871

来源: DOAJ

【 摘 要 】

A cross-sectional study was performed in 325 sheep and 119 goat dairy farms in Greece. Samples of bulk-tank milk were examined by standard microbiological techniques for Listeria spp. Listeria monocytogenes was isolated from one (0.3%) and Listeria ivanovii from three (0.9%) sheep farms. No associations between the isolation of L. monocytogenes or L. ivanovii and milk quality were found. No resistance to antibiotics was identified. Three variables emerged as significant predictors of isolation of the organism: the presence of pigs, low average relative humidity and a high number of ewes on the farm. The three L. ivanovii isolates were assessed in silico for identification of plasmids, prophages, antibiotic resistance genes, virulence factors, CRISPRs and CAS genes. Phylogenetic analysis using the core genome revealed that the three strains belonged to the L. ivanovii subsp. ivanovii branch and were especially close to the PAM 55 strain. All strains of the branch appeared to be very similar, with the distance between them being small.

【 授权许可】

Unknown