High-throughput sequencing technology reveals polysaccharides from Angelica dahurica that affect gut microbiota in mice

Biotechnology & Biotechnological Equipment

Hong Niu¹  Qi Fang¹  Yu Luo¹  Yong Lai¹  Rui Wang¹  Can Song¹ 

[1] Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, PR China;

关键词: High throughput sequencing;    polysaccharides;    gut microbiota;    Angelica dahurica;   

DOI  :  10.1080/13102818.2022.2045216

来源: DOAJ

【 摘 要 】

Angelica dahurica is an ancient herbaceous plant and a famous traditional Chinese medicine. A. dahurica polysaccharides (AdPs) are macromolecular bioactive carbohydrates that have many functions. The animal gut contains a remarkable abundance of microbes that are closely related and play a major role in maintaining the overall health. The intestinal flora is also known as the ‘second genome’ of the human body. To study the functional interactions between AdPs and gut microbiota, we extracted AdPs using water extraction. After a series of treatments, including degreasing, alcohol precipitation, protein decolorization and purification by cellulose column, 200 mg/kg of purified AdPs were administered daily to 5 weeks old Kunming (KM) mice for 21 days. Microbial composition was evaluated in fecal samples using high-throughput sequencing. Our results revealed that AdPs affected the composition and structure of gut microbiota and can potentially regulate the intestinal flora as prebiotics.

【 授权许可】

Unknown