The impact of COL1A1 and COL6A1 expression on hypospadias and penile curvature severity

BMC Urology

Nur Arfian¹  Wiwit Ananda Wahyu Setyaningsih¹  Ishandono Dachlan²  Firly Putri Fardilla³  Prahara Yuri³  Rahmadani Puji Lestari³  Gunadi⁴ 

[1] Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada;Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine,Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital;Division of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital;Pediatric Surgery Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital;

关键词: Chordee;    COL1A1;    COL6A1;    Expressions;    Hypospadias;    Penile curvature severity;   

DOI  :  10.1186/s12894-020-00760-w

来源: DOAJ

【 摘 要 】

Abstract Background Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of darto tissue has been suggested as the pathophysiology of chordees. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expression of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature. Methods We included 60 children < 18 years old, consisting of 20 distal hypospadias, 20 proximal hypospadias patients, and 20 controls in our institution from 2017 – 2020. The expression of COL1A1 and COL6A1 in darto tissue was determined by reverse-transcriptase polymerase chain reaction (qPCR). The penile curvature severity was classified as mild (< 30 degrees), moderate (30–60 degrees), and severe (> 60 degrees). Results qPCR showed that COL1A1 and COL6A1 expression was significantly downregulated in the distal (0.88 (0.38–2.53) and 0.54 (0.16–4.35), respectively) and proximal 0.76 (0.33–2.57) and 0.57 (0.18–1.38), respectively) hypospadias groups compared to controls (1.85 (0.24–4.61) and 0.93 (0.17–4.06), respectively) with p-values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expression (r = 0.458, p < 0.0001). Interestingly, COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe chordee groups compared to the mild chordee groups, with p-values of 0.003 and 0.037, respectively. Conclusions Aberrant COL1A1 and COL6A1 expression might affect abnormalities in darto tissue and penile curvature severity in hypospadias patients.

【 授权许可】

Unknown