Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

International Journal of Molecular Sciences

JaimeA. Teixeira da Silva¹  Zhenming Yu²  Danqi Zeng²  Jun Duan²  Mingze Zhang²  Chunmei He²  Haobin Wang²  Conghui Zhao²  Can Si² 

[1] Independent Researcher, P.O. Box 7, Miki-Cho Post Office, Ikenobe 3011-2, Kagawa-ken 761-0799, Japan;Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;

关键词: floral volatiles;    geraniol;    MEP pathway;    orchids;    terpene synthase;   

DOI  :  10.3390/ijms21197005

来源: DOAJ

【 摘 要 】

Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids.

【 授权许可】

Unknown