SPEM: a state-of-the-art instrument for high resolution molecular imaging of small animal organs

Einstein (São Paulo)

Ilza Rosa Batista¹  Rodrigo Affonseca Bressan¹  Edson Amaro Junior¹  Ming Chi Shih¹  Petrick Marcellus Victorio Mendonça Davoglio¹  Jairo Wagner¹  Marycel Rosa Felisa Figols de Barboza¹  Jorge Mejia¹  Marilia Alves dos Reis¹  Francisco Romero Cabral¹  Solange Amorim Nogueira¹  Vanessa Costhek Abílio²  Chin-Tu Chen³  Nan Li⁴  Ling Jian Meng⁴  Geng Fu⁴ 

[1] Hospital Israelita Albert Einstein;Universidade Federal de São Paulo;University of Chicago;University of Illinois;

关键词: Molecular imaging;    Pre-clinical research;    Small animals;    Single photon emission computed tomography;    Multipinhole collimation;   

DOI  :  

来源: DOAJ

【 摘 要 】

OBJECTIVE: To describe the Single Photon Emission Microscope (SPEM), a state-of-the-art instrument for small animal SPECT imaging, and characterize its performance presenting typical images of different animal organs. METHODS: SPEM consists of two independent imaging devices based on high resolution scintillators, high sensitivity and resolution Electron-Multiplying CCDs and multi-pinhole collimators. During image acquisition, the mouse is placed in a rotational vertical holder between the imaging devices. Subsequently, an appropriate software tool based on the Maximum Likelihood algorithm iteratively produces the volumetric image. Radiopharmaceuticals for imaging kidneys, heart, thyroid and brain were used. The mice were injected with 74 to 148 MBq/0,3mL and scanned for 40 to 80 minutes, 30 to 60 minutes afterwards. During this procedure, the animals remained under ketamine/xilazine anesthesia. RESULTS: SPEM images of different mouse organs are presented, attesting the imaging capabilities of the instrument. CONCLUSION: SPEM is an innovative technology for small animal SPECT imaging providing high resolution images with appropriate sensitivity for pre-clinical research. Its use with appropriate radiotracers will allow translational investigation of several animal models of human diseases, their pharmacological treatment and the development of potential new therapeutic agents.

【 授权许可】

Unknown