Phage Display Screening of Bovine Antibodies to Foot-and-Mouth Disease Virus and Their Application in a Competitive ELISA for Serodiagnosis

International Journal of Molecular Sciences

Hyun-Mi Pyo¹  Mi-Young Park¹  Bok-Kyung Ku¹  Sung-Hwan Wee¹  Jae-Won Byun¹  Jinju Nah¹  Soyoon Ryoo¹  Kyung-Jin Min²  Hyun-Joo Ahn²  Sang-Jick Kim²  Sukyo Jeong² 

[1] Foot-and-Mouth Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea;Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea;

关键词: foot-and-mouth disease virus;    type O;    type A;    phage display;    antibody;    competitive ELISA;   

DOI  :  10.3390/ijms22094328

来源: DOAJ

【 摘 要 】

For serodiagnosis of foot-and-mouth disease virus (FMDV), monoclonal antibody (MAb)-based competitive ELISA (cELISA) is commonly used since it allows simple and reproducible detection of antibody response to FMDV. However, the use of mouse-origin MAb as a detection reagent is questionable, as antibody responses to FMDV in mice may differ in epitope structure and preference from those in natural hosts such as cattle and pigs. To take advantage of natural host-derived antibodies, a phage-displayed scFv library was constructed from FMDV-immune cattle and subjected to two separate pannings against inactivated FMDV type O and A. Subsequent ELISA screening revealed high-affinity scFv antibodies specific to a serotype (O or A) as well as those with pan-serotype specificity. When BvO17, an scFv antibody specific to FMDV type O, was tested as a detection reagent in cELISA, it successfully detected FMDV type O antibodies for both serum samples from vaccinated cattle and virus-challenged pigs with even higher sensitivity than a mouse MAb-based commercial FMDV type O antibody detection kit. These results demonstrate the feasibility of using natural host-derived antibodies such as bovine scFv instead of mouse MAb in cELISA for serological detection of antibody response to FMDV in the susceptible animals.

【 授权许可】

Unknown