Optimization of the Ultrasound-Assisted Extraction of Phenolic Compounds from Brosimum alicastrum Leaves and the Evaluation of Their Radical-Scavenging Activity

Molecules

Mariel Gullian Klanian¹  Montserrat Terrats Preciat¹ 

[1] Experimental Unit, University Marist of Mérida, Periférico Norte Tablaje Catastral 13941, Carretera Mérida-Progreso, 97300 Mérida, Yucatán, Mexico;

关键词: Brosimum alicastrum leaf;    ultrasound assisted extraction;    response surface methodology;    total phenolic content;    monomeric anthocyanin;    radical-scavenging activity;   

DOI  :  10.3390/molecules22081286

来源: DOAJ

【 摘 要 】

In order to maximize the yield of the total phenolic content (TPC) and total monomeric anthocyanin (TMA) from Brosimum alicastrum leaf and to study the radical-scavenging activity, a three-level three-factor Box–Behnken design (BBD) was used to determine the optimal points for ultrasound-assisted extraction (UAE). In this study, we analyzed the extraction time (10, 20, and 30 min), temperature (28, 30, and 32 °C), and probe sonication power (40%, 28 W/cm2; 60%, 51 W/cm2; and 80%, 74 W/cm2). Analysis of variance (ANOVA) indicated that the sonication power plays a significant role in the extraction of phenolic compounds. An increase in time and temperature resulted in a decrease in the yield, in particular, of the TMA group. DPPH was found to be a better indicator of radical-scavenging activity than ABTS. The predicted TPC and TMA optimum levels (45.18 mg GAE/g and 15.16 mg CyE/100 g) were obtained at 28 °C, 80%, and 20–10 min. DPPH obtained a maximum value (67.27 μmol TE/g) under same optimization conditions. The RSM confirmed that TPC and TMA enhanced the antioxidant activity when subjected to low temperature (28 °C), extraction time less than 20 min, and higher sonication power (74 W/cm2), and hence achieving the better DPPH scavenging activity.

【 授权许可】

Unknown