IDENTIFICATION OF BETA-LACTOGLOBULIN AND KAPPACASEIN GENOTYPES IN CATTLE

Scientific Papers Animal Science and Biotechnologies

C.D. TESIO¹  S.E. GEORGESCU¹  ANCA DINISCHIOTU¹  MARIA ADINA MANEA¹  MARIETA COSTACHE¹  R.A. VĂTĂŞESCU-BALCAN¹ 

[1] University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest;

关键词: kappa-casein;    beta-lactoglobulin;    genetic polymorphism;    pcr-rflp test;   

DOI  :  

来源: DOAJ

【 摘 要 】

Beta-lactoglobulin (b-Lg) and kappa-casein (k-Cn) are two of the most important proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands. They play a crucial role in the milk quality and coagulation process (production of cheese and butter). The PCR-RFLP test was performed to distinguish the different alleles in a population of Romanian Black Spotted cattle, a dairy breed. Genetic polymorphism was detected by digestion with the endonucleases Hae III (b-Lg) and Hinf I (k-Cn), followed by electrophoresis in agarose high resolution gel stained with ethidium bromide. Fifty DNA samples from Romanian Black Spotted breed were analyzed for A and B variants. This simple PCR-RFLP test makes feasible the inclusion of b-Lg and k- Cn genotypes in breeding plans and cattle selection.

【 授权许可】

Unknown