Aromatic Hydrocarbon Removal by Novel Extremotolerant Exophiala and Rhodotorula Spp. from an Oil Polluted Site in Mexico

Journal of Fungi

Alfonso Leija¹  Maríadel Rayo Sánchez-Carbente²  MartínR. Ide-Pérez²  JorgeLuis Folch-Mallol²  RamónAlberto Batista-García³  MaikelGilberto Fernández-López³  Ayixon Sánchez-Reyes⁴ 

[1] Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62209, Mexico;Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico;Centro de Investigación en Dinámica Celular-Instituto de Investigaciones Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico;Cátedras Conacyt-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62209, Mexico;

关键词: Exophiala sp.;    Rhodotorula sp.;    dimorphism;    aromatic hydrocarbons;    phenanthrene;    benzo[a]pyrene;   

DOI  :  10.3390/jof6030135

来源: DOAJ

【 摘 要 】

Since Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular markers as a novel Rhodotorula sp. and an Exophiala sp. Both strains were able to grow in a wide range of pH media, from 4 to 12, showing their optimal growth at alkaline pH’s and are both halotolerant. The Exophiala strain switched from hyphae to yeast morphotype in high salinity conditions. To the best of our knowledge, this is the first report of salt triggering dimorphism. The Rhodotorula strain, which is likely a new undescribed species, was capable of removing singled ringed aromatic compounds such as benzene, xylene, and toluene, but could not remove benzo[a] pyrene nor phenanthrene. Nevertheless, these hydrocarbons did not impair its growth. The Exophiala strain showed a different removal capacity. It could remove the polyaromatic hydrocarbons but performed poorly at removing toluene and xylene. Nevertheless, it still could grow well in the presence of the aromatic compounds. These strains could have a potential for aromatic compounds removal.

【 授权许可】

Unknown