Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

Microorganisms

Sulaiman A. Alrumman¹  Ahmed A. M. Shoreit²  Amal W. Danial²  Sanaa M. F. Gad El-Rab²  Abd El-Latif Hesham³  Shereen M. Hamdy⁴ 

[1] Biology Department, College of Science, King Khalid University, Abha 61413, Saudi Arabia;Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt;Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni Suef 62521, Egypt;Pediatric Hospital, Assiut University, Assiut 71516, Egypt;

关键词: Polyhydroxybutyrates (PHBs);    agricultural wastes;    bioplastic;    bacteria;    16S rRNA analysis;    NMR;   

DOI  :  10.3390/microorganisms9112395

来源: DOAJ

【 摘 要 】

Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their ability to accumulate PHB granules inside their cells. Isolate AS-02 was isolated from cattle manure and identified as Bacillus wiedmannii AS-02 OK576278 by means of 16S rRNA analysis. It was found to be the best producer. The optimum pH, temperature, and incubation period for the best PHB production by the isolate were 7, 35 °C, and 72 h respectively. PHB production was the best with peptone and glucose as nitrogen and carbon sources at a C/N ratio of (2:1). The strain was able to accumulate 423, 390, 249, 158, and 144 mg/L PHB when pretreated orange, mango, banana, onion peels, and rice straw were used as carbon sources, respectively. The extracted polymer was characterized by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. The isolate B. wiedmannii AS-02 OK576278 can be considered an excellent candidate for industrial production of PHB from agricultural wastes.

【 授权许可】

Unknown