Overview of Recent Advances in Immobilisation Techniques for Phenol Oxidases in Solution

Catalysts

Thandanani Ndlovu¹  SorayaP Malinga¹  Sidy Ba² 

[1] Department of Chemical Sciences, University of Johannesburg, Doornfontein 2028, South Africa;Department of Rural Engineering and Water & Forest, Institut Polytechnique Rural de Formation et de Recherche Appliquée de Katibougou, Koulikoro BP 06, Mali;

关键词: immobilisation techniques;    inhibitors;    laccases;    stability;    tyrosinases;    wastewater decontamination;   

DOI  :  10.3390/catal10050467

来源: DOAJ

【 摘 要 】

Over the past two decades, phenol oxidases, particularly laccases and tyrosinases, have been extensively used for the removal of numerous pollutants in wastewaters due to their broad substrate specificity and their ability to use readily accessible molecular oxygen as the essential cofactor. As for other enzymes, immobilisation of laccases and tyrosinases has been shown to improve the performance and efficiency of the biocatalysts in solution. Several reviews have addressed the enzyme immobilisation techniques and the application of phenol oxidases to decontaminate wastewaters. This paper offers an overview of the recent publications, mainly from 2012 onwards, on the various immobilisation techniques applied to laccases and tyrosinases to induce and/or increase the performance of the biocatalysts. In this paper, the emphasis is on the efficiencies achieved, in terms of structural modifications, stability and resistance to extreme conditions (pH, temperature, inhibitors, etc.), reactivity, reusability, and broad substrate specificity, particularly for application in bioremediation processes. The advantages and disadvantages of several enzyme immobilisation techniques are also discussed. The relevance and effectiveness of the immobilisation techniques with respect to wastewater decontamination are critically assessed. A perspective on the future directions for large-scale application of the phenol oxidases in immobilised forms is provided.

【 授权许可】

Unknown