bla NDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29

Antimicrobial Resistance and Infection Control

Chengwen Li¹  Ying Li¹  Yi Yuan²  Fangcai Zhong²  Guangxi Wang³  Siji Nian³  Yingyu Mao³  Jinping Zhang³  Luhua Zhang³  Wenbi Chen³  Yung-Fu Chang⁴ 

[1] Department of Immunology, School of Basic Medical Sciences, Southwest Medical University;Department of Laboratory Medicine, The First People’s Hospital of Neijiang;Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University;Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University;

关键词: Carbapenem resistance;    bla NDM-5;    Hypervirulent;    IncX3;   

DOI  :  10.1186/s13756-019-0596-1

来源: DOAJ

【 摘 要 】

Abstract Background A carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum. Methods Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays were performed using wax moth larvae. The transfer of bla NDM-5 between bacterial strains was tested using conjugation. 59 genome assemblies of ST29 K. pneumoniae and 230 IncX3 plasmids regardless of the carriage of resistance gene were employed for phylogenetic analysis, respectively. Results The strain carried a virulence plasmid pVir-SCNJ1 bearing the virulence gene rmpA and exhibited a high virulence in wax moth. This hypervirulent strain belongs to sequence type 29 and carries bla NDM-5, which is located on a conjugative plasmid, designated pNDM5-SCNJ1, belonging to type IncX3. pNDM5-SCNJ1 was fully sequenced and shows high similarity with pNDM_MGR194, except some deletion inside the ISAba125 region. Phylogenetic analysis of IncX3 plasmids revealed that although bla NDM-5 can be evolved from bla NDM-1 via point mutations within some IncX3 plasmids, most of bla NDM-5-carrying IncX3 plasmids probably have acquired bla NDM-5 in multiple events. Conclusions In this study, we characterized a bla NDM-5-positive hypervirulent K. pneumoniae of sequence type 29 in China. Our results highlight the need for active surveillance on this lineage of carbapenem-resistant K. pneumoniae.

【 授权许可】

Unknown