Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia

Viruses

HetronM. Munang’andu¹  Yongjin Qiu²  Yuki Eshita²  Alikhadio Maseko³  Paul Fandamu³  Yona Sinkala³  Choopa Chimvwele³  Liywalii Mataa³  CaesarH. Lubaba³  Gabriel Gonzalez⁴  MichaelJ. Carr⁴  WilliamW. Hall⁴  HermanM. Chambaro⁵  Yasuko Orba⁵  Michihito Sasaki⁵  Hayato Harima⁵  Hirofumi Sawa⁵  Musso Munyeme⁶  Martin Simuunza⁶  Edgar Simulundu⁶  KennyL. Samui⁶  AndrewN. Mukubesa⁶  Isaac Silwamba⁶  KingS. Nalubamba⁶  David Squarre⁷  LynnfieldE. Mooya⁸ 

[1] Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, 0454 Oslo, Norway;Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia;Ministry of Fisheries and Livestock, Lusaka 10101, Zambia;National Virus Reference Laboratory, School of Medicine, Dublin DO4V1W8, Ireland;Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan;School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia;The University of Edinburgh, Edinburgh EH25 9RG, Scotland, UK;Virology Unit, Central Veterinary Research Institute, Lusaka 10101, Zambia;

关键词: Reoviridae;    bluetongue;    bluetongue virus;    serotypes;    topotypes;    sero-surveillance;   

DOI  :  10.3390/v12090963

来源: DOAJ

【 摘 要 】

Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillance for BT in domestic and wild ruminants in Zambia, BT virus (BTV) nucleic acid and antibodies were detected in eight of the 10 provinces of the country. About 40% (87/215) of pooled blood samples from cattle and goats were positive for BTV nucleic acid, while one hartebeest pool (1/43) was positive among wildlife samples. Sequence analysis of segment 2 revealed presence of serotypes 3, 5, 7, 12 and 15, with five nucleotypes (B, E, F, G and J) being identified. Segment 10 phylogeny showed Zambian BTV sequences clustering with Western topotype strains from South Africa, intimating likely transboundary spread of BTV in Southern Africa. Interestingly, two Zambian viruses and one isolate from Israel formed a novel clade, which we designated as Western topotype 4. The high seroprevalence (96.2%) in cattle from Lusaka and Central provinces and co-circulation of multiple serotypes showed that BT is widespread, underscoring the need for prevention and control strategies.

【 授权许可】

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