Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays

Journal of Fungi

Sara Mayo-Prieto¹  Álvaro Rodríguez-González¹  PedroA. Casquero¹  Samuel Álvarez-García¹  Guzmán Carro-Huerga¹  Óscar González-López¹  Santiago Gutiérrez² 

[1] Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Avenida Portugal 41, 24071 Leon, Spain;Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Área de Microbiología, Escuela de Ingeniería Agraria y Forestal, Universidad de León, Campus de Ponferrada, Avenida Astorga s/n, 24401 Ponferrada, Spain;

关键词: volatile organic compounds;    VOCs;    trichodiene;    squalene;    Trichoderma;    Fusarium;   

DOI  :  10.3390/jof7040248

来源: DOAJ

【 摘 要 】

The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the volatile organic compound chamber (VOC chamber), specifically designed to perform these experiments. The novel devices were tested using four Trichoderma strains against Fusarium oxysporum and Rhizoctonia solani. We demonstrate that VOC chambers provide higher sensitivity and selectivity between treatments and higher homogeneity of results than the traditional method. VOC chambers are also able to test both vented and non-vented conditions. We prove that ventilation plays a very important role regarding volatile interactions, up to the point that some growth-inhibitory effects observed in closed environments switch to promoting ones when tested in vented conditions. This promoting activity seems to be related to the accumulation of squalene by T. harzianum. The VOC chambers proved to be an easy, homogeneous, flexible, and repeatable method, able to better select microorganisms with high biocontrol activity and to guide the future identification of new bioactive VOCs and their role in microbial interactions.

【 授权许可】

Unknown