High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis

BMC Genomics

Wanxin Wang¹  Derek Croote¹  Lolita Penland¹  Stephen R. Quake¹  Ozgun Gokce² 

[1] Department of Bioengineering, Stanford University, James H Clark Center, E300, 318 Campus Drive;Department of Molecular and Cellular Physiology, Stanford University;

关键词: Single cell RNAseq;    Primary tissue preservation without dissociation;    Hypothermic preservation;    Organ transplant preservation;    Organ transplant preservative;    Kidney resident immune cells;   

DOI  :  10.1186/s12864-018-4512-5

来源: DOAJ

【 摘 要 】

Abstract Background High-fidelity preservation strategies for primary tissues are in great demand in the single cell RNAseq community. A reliable method would greatly expand the scope of feasible multi-site collaborations and maximize the utilization of technical expertise. When choosing a method, standardizability and fidelity are important factors to consider due to the susceptibility of single-cell RNAseq analysis to technical noise. Existing approaches such as cryopreservation and chemical fixation are less than ideal for failing to satisfy either or both of these standards. Results Here we propose a new strategy that leverages preservation schemes developed for organ transplantation. We evaluated the strategy by storing intact mouse kidneys in organ transplant preservative solution at hypothermic temperature for up to 4 days (6 h, 1, 2, 3, and 4 days), and comparing the quality of preserved and fresh samples using FACS and single cell RNAseq. We demonstrate that the strategy effectively maintained cell viability, transcriptome integrity, cell population heterogeneity, and transcriptome landscape stability for samples after up to 3 days of preservation. The strategy also facilitated the definition of the diverse spectrum of kidney resident immune cells, to our knowledge the first time at single cell resolution. Conclusions Hypothermic storage of intact primary tissues in organ transplant preservative maintains the quality and stability of the transcriptome of cells for single cell RNAseq analysis. The strategy is readily generalizable to primary specimens from other tissue types for single cell RNAseq analysis.

【 授权许可】

Unknown