Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction

Romanian Journal of Laboratory Medicine

Khan Saif¹  Farooqui Alvina²  Khan Mahvish³  Husain Arbab⁴  Khanam Afreen⁴  Ahmad Saheem⁵  Akasha Rihab⁵  Alouffi Sultan⁵ 

[1] Department of Basic Dental and Medical Sciences, College of Dentistry, University of Hail, Saudi Arabia;Department of Bioengineering, Integral University, India;Department of Biology, College of Science, University of Hail, Saudi Arabia;Department of Biosciences, Integral University, India;Department of Medical Laboratory Sciences, College of Applied Medical Sciences,, University of Hail, Saudi Arabia;

关键词: glycation;    antioxidant;    antiglycation;    plectonema sp.;    c-phycocyanin;    bovine serum albumin (bsa);    methylglyoxal (mg);   

DOI  :  10.2478/rrlm-2022-0018

来源: DOAJ

【 摘 要 】

When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible & fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine & free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.

【 授权许可】

Unknown