Electrospinning Live Cells Using Gelatin and Pullulan

Bioengineering

Nasim Nosoudi¹  AnsonJacob Oommen²  Seba Aldabel²  Alliah Turner²  James Mosser²  Chandra Hohne²  Paul Turner²  Savannah Stultz²  Bailey Archacki²  Micah Jordan² 

[1] Department of biomedical engineering, College of Engineering and Computer Sciences (CECS), Marshall University, Weisberg Family Applied Engineering Complex, Huntington, WV 25755, USA;Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA;

关键词: electrospinning;    live-cell electrospinning;    tissue engineering;    cell seeding;    high voltage;    viability;   

DOI  :  10.3390/bioengineering7010021

来源: DOAJ

【 摘 要 】

Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biological constitutes are implanted into the matrix of the three-dimensional scaffold following electrospinning. Our design simultaneously introduces cells into the scaffold during the electrospinning process at 8 kV. In this study, we achieved 90% viability of adipose tissue-derived stem cells through electrospinning.

【 授权许可】

Unknown