(Phospho)Proteomic dataset of ischemia- and ultrasound- stimulated mouse cardiac endothelial cells in vitro

Data in Brief

Nabil J. Alkayed¹  Jason E. McDermott²  Kristin L. Scott²  David Giraud²  Anthony P. Barnes²  Sanjiv Kaul³  Catherine M. Davis³  Igor V. Dykan⁴  Jon M. Jacobs⁵  Azzdine Y. Ammi⁵  Uchenna Emechebe⁵ 

[1] Perioperative Medicine, Oregon Health &Science University, Portland, OR, 97239, USA;Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, USA;;Department of Anesthesiology &;Knight Cardiovascular Institute, Oregon Health &

关键词: Ultrasound;    Heart;    Endothelial cell;    Ischemia;    Proteomics;    Phosphoproteomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Cardiac endothelial cells respond to both ischemia and therapeutic ultrasound; the proteomic changes underlying these responses are unknown. This data article provides raw and processed data resulting from our global, unbiased phosphoproteomics investigation conducted on primary mouse cardiac endothelial cells exposed to ischemia (2-hour oxygen glucose deprivation) and ultrasound (250 kHz, 1.2 MPa) in vitro [1]. Proteins were extracted from cell lysates and enriched phosphopeptides were analyzed with a high mass accuracy liquid chromatrography (LC) - tandem mass spectrometry (MS/MS) proteomic platform, yielding multiple alterations in both total protein levels and phosphorylation events in response to ischemic injury and ultrasound. This dataset can be used as a reference for future studies on the cardiac endothelial response to ischemia and the mechanistic underpinnings of the cellular response to ultrasound, with the potential to yield clinically relevant therapeutic targets.

【 授权许可】

Unknown