Impacts of Endocrine Disruptor di-n-Butyl Phthalate Ester on Microalga Chlorella vulgaris Verified by Approaches of Proteomics and Gene Ontology

Molecules

Chien-Sen Liao¹  Yong-Han Hong²  Yoshikazu Nishikawa³  Eriko Kage-Nakadai³  Chien-Chang Wu⁴  Tai-Ying Chiou⁵ 

[1] Department of Biological Science and Technology, I Shou University, Kaohsiung 82445, Taiwan;Department of Nutrition, I Shou University, Kaohsiung 84001, Taiwan;Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan;Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan;School of Regional Innovation and Social Design Engineering, Kitami Institute of Technology, Hokkaido 090-8507, Japan;

关键词: Chlorella vulgaris;    di-n-butyl phthalate;    algal acute biotoxicity;    proteomics;    gene ontology;   

DOI  :  10.3390/molecules25184304

来源: DOAJ

【 摘 要 】

Di-n-butyl phthalate (DBP) is an extensively used plasticizer. Most investigations on DBP have been concentrated on its environmental distribution and toxicity to humans. However, information on the effects of plasticizers on algal species is scarce. This study verified the impacts of endocrine disruptor di-n-butyl phthalate ester on microalga Chlorella vulgaris by approaches of proteomics and gene ontology. The algal acute biotoxicity results showed that the 24h-EC50 of DBP for C. vulgaris was 4.95 mg L⁻¹, which caused a decrease in the chlorophyll a content and an increase in the DBP concentration of C. vulgaris. Proteomic analysis led to the identification of 1257 C. vulgaris proteins. Sixty-one more proteins showed increased expression, compared to proteins with decreased expression. This result illustrates that exposure to DBP generally enhances protein expression in C. vulgaris. GO annotation showed that both acetolactate synthase (ALS) and GDP-L-fucose synthase 2 (GER2) decreased more than 1.5-fold after exposure to DBP. These effects could inhibit both the valine biosynthetic process and the nucleotide-sugar metabolic process in C. vulgaris. The results of this study demonstrate that DBP could inhibit growth and cause significant changes to the biosynthesis-relevant proteins in C. vulgaris.

【 授权许可】

Unknown