Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana

eLife

Veronique Storme¹  Mattias Vermeersch¹  Liesbeth De Milde¹  Nathalie Gonzalez¹  Hannes Vanhaeren²  Twiggy Van Daele²  Frederik Coppens²  Nubia B Eloy²  Dirk Inzé² 

[1] Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, Ghent, Belgium;

关键词: leaf growth;    gene interaction;    organ size control;    cell division;    cell expansion;    epistasis;   

DOI  :  10.7554/eLife.02252

来源: DOAJ

【 摘 要 】

Several genes positively influence final leaf size in Arabidopsis when mutated or overexpressed. The connections between these growth regulators are still poorly understood although such knowledge would further contribute to understand the processes driving leaf growth. In this study, we performed a combinatorial screen with 13 transgenic Arabidopsis lines with an increased leaf size. We found that from 61 analyzed combinations, 39% showed an additional increase in leaf size and most resulted from a positive epistasis on growth. Similar to what is found in other organisms in which such an epistasis assay was performed, only few genes were highly connected in synergistic combinations as we observed a positive epistasis in the majority of the combinations with samba, BRI1OE or SAUR19OE. Furthermore, positive epistasis was found with combinations of genes with a similar mode of action, but also with genes which affect distinct processes, such as cell proliferation and cell expansion.

【 授权许可】

Unknown