Interaction between Plasmodium Glycosylphosphatidylinositol and the Host Protein Moesin Has No Implication in Malaria Pathology

Frontiers in Cellular and Infection Microbiology

Peter H. Seeberger¹  Nahid Azzouz¹  Xinyu Liu²  Sachiko Tsukita³  Josefine Dunst⁵  Faustin Kamena⁵ 

[1] Department of Biomolecular Systems, Max Planck Institute for Colloids and InterfacesPotsdam, Germany;Department of Chemistry, University of PittsburghPittsburgh, PA, USA;Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka UniversityOsaka, Japan;Institute of Chemistry and Biochemistry, Free University BerlinBerlin, Germany;Parasitology Unit, Max Planck Institute for Infection BiologyBerlin, Germany;

关键词: GPI;    ERM;    moesin;    Plasmodium;    cerebral malaria;   

DOI  :  10.3389/fcimb.2017.00183

来源: DOAJ

【 摘 要 】

Glycosylphosphatidylinositol (GPI) anchor of Plasmodium falciparum origin is considered an important toxin leading to severe malaria pathology through stimulation of pro-inflammatory responses from innate immune cells. Even though the GPI-induced immune response is widely described to be mediated by pattern recognition receptors such as TLR2 and TLR4, previous studies have revealed that these two receptors are dispensable for the development of severe malaria pathology. Therefore, this study aimed at the identification of potential alternative Plasmodium GPI receptors. Herein, we have identified the host protein moesin as an interaction partner of Plasmodium GPI in vitro. Given previous reports indicating the relevance of moesin especially in the LPS-mediated induction of pro-inflammatory responses, we have conducted a series of in vitro and in vivo experiments to address the physiological relevance of the moesin-Plasmodium GPI interaction in the context of malaria pathology. We report here that although moesin and Plasmodium GPI interact in vitro, moesin is not critically involved in processes leading to Plasmodium-induced pro-inflammatory immune responses or malaria-associated cerebral pathology.

【 授权许可】

Unknown