Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body

eLife

Remy Tabano¹  Christina Christoforou¹  Shahar Frechter²  Davi D Bock³  Philipp Schlegel³  Marta Costa⁴  Chuntao Dan⁴  Serene Dhawan⁴  Ruairí JV Roberts⁴  Alexander Shakeel Bates⁴  Heather Dionne⁴  Kari Close⁵  Geoffrey Wilson Meissner⁵  Gregory SXE Jefferis⁵  Michael-John Dolan⁵  Paavo Huoviala⁵  Feng Li⁵  Ben Sutcliffe⁵  Gudrun Ihrke⁵  Bianca Giuliani⁵  Gerald M Rubin⁵  Yoshinori Aso⁵ 

[1]Department of Zoology, University of Cambridge, Cambridge, United Kingdom

[2]Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

[3]Department of Zoology, University of Cambridge, Cambridge, United Kingdom

[4]Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

[5]Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States

关键词: memory;    learning;    lateral Horn;    innate;    behaviour;    neurogenetics;   

DOI  :  10.7554/eLife.43079

来源: DOAJ

【 摘 要 】

Animals exhibit innate behaviours to a variety of sensory stimuli including olfactory cues. In Drosophila, one higher olfactory centre, the lateral horn (LH), is implicated in innate behaviour. However, our structural and functional understanding of the LH is scant, in large part due to a lack of sparse neurogenetic tools for this region. We generate a collection of split-GAL4 driver lines providing genetic access to 82 LH cell types. We use these to create an anatomical and neurotransmitter map of the LH and link this to EM connectomics data. We find ~30% of LH projections converge with outputs from the mushroom body, site of olfactory learning and memory. Using optogenetic activation, we identify LH cell types that drive changes in valence behavior or specific locomotor programs. In summary, we have generated a resource for manipulating and mapping LH neurons, providing new insights into the circuit basis of innate and learned olfactory behavior.

【 授权许可】

Unknown