Short-Term Exposure of Mytilus coruscus to Decreased pH and Salinity Change Impacts Immune Parameters of Their Haemocytes

Frontiers in Physiology

Sam Dupont¹  Weiqun Lu³  Shuaikang Cui³  Fangli Wu³  Liping Liu³  Menghong Hu³  Yawen Lan³  Meng Sun³  Xizhi Huang³  Zhe Xie³  Wei Huang⁴  Youji Wang⁶ 

[1] Department of Biological and Environmental Sciences, Sven Lovén Centre for Marine Infrastructure-Kristineberg, University of Gothenburg, Fiskebäckskil, Sweden;International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China;Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China;State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, China;

关键词: acidification;    salinity;    Mytilus coruscus;    flow cytometry;    immune response;    haemocyte;   

DOI  :  10.3389/fphys.2018.00166

来源: DOAJ

【 摘 要 】

With the release of large amounts of CO2, ocean acidification is intensifying and affecting aquatic organisms. In addition, salinity also plays an important role for marine organisms and fluctuates greatly in estuarine and coastal ecosystem, where ocean acidification frequently occurs. In present study, flow cytometry was used to investigate immune parameters of haemocytes in the thick shell mussel Mytilus coruscus exposed to different salinities (15, 25, and 35‰) and two pH levels (7.3 and 8.1). A 7-day in vivo and a 5-h in vitro experiments were performed. In both experiments, low pH had significant effects on all tested immune parameters. When exposed to decreased pH, total haemocyte count (THC), phagocytosis (Pha), esterase (Est), and lysosomal content (Lyso) were significantly decreased, whereas haemocyte mortality (HM) and reactive oxygen species (ROS) were increased. High salinity had no significant effects on the immune parameters of haemocytes as compared with low salinity. However, an interaction between pH and salinity was observed in both experiments for most tested haemocyte parameters. This study showed that high salinity, low salinity and low pH have negative and interactive effects on haemocytes of mussels. As a consequence, it can be expected that the combined effect of low pH and changed salinity will have more severe effects on mussel health than predicted by single exposure.

【 授权许可】

Unknown