Assessment of E. coli Expression System for Overexpression of Active Recombinant Ocriplasmin

Advanced Pharmaceutical Bulletin

Safar Farajnia¹  Mojtaba Mortazavi²  Younes Ghasemi³  Naser Samadi⁴  Nosratollah Zarghami⁵  Roghayyeh Baghban⁵ 

[1] Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;Department of Biotechnology, Institute of Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran.;Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Science, Shiraz, Iran.;Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.;Medical Biotechnology Department, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences,Tabriz, Iran.;

关键词: ocriplasmin;    recombinant expression;    vitreomacular adhesion (vma);   

DOI  :  10.34172/apb.2021.065

来源: DOAJ

【 摘 要 】

Purpose: Ocriplasmin (Jetrea TM) is a FDA approved recombinant enzyme utilized in the treatment of vitreomacular adhesion (VMA). This is a recombinant C-terminal fragment of human plasmin produced using yeast Pichia pastoris. Since ocriplasmin does not contain any Oor N-glycosylation or some other post-translational modifications, bacterial expression systems such as Escherichia coli could be considered as an economical host for recombinant expression. In the present study, we aimed to evaluate the efficiency of E. coli expression system for high-level expression of recombinant ocriplasmin. Methods: The gene coding for ocriplasmin was cloned and expressed in E. coli BL21. The bacterial cells were cultured on large scale and the expressed recombinant protein was purified using Ni-NTA chromatography. Refolding of denatured ocriplasmin to active enzyme was carried out by the stepwise removal of denaturant. The identity of recombinant ocriplasmin was confirmed using western blotting and ELISA assays. The presence of the active ocriplasmin was monitored by the hydrolytic activity assay against the chromogenic substrate S-2403. Results: The final yield of E. coli BL21-produced ocriplasmin was approximately 1 mg/mL which was greater than that of P. pastoris. Using western blotting and ELISA assay, the identity of recombinant ocriplasmin was confirmed. The hydrolysis of chromogenic substrate S-2403 verified the functional activity of E. coli produced ocriplasmin. Conclusion: The results of this study indicated that E. coli could be used for high level expression of ocriplasmin. Although the recombinant protein was expressed as inclusion body, the stepwise refolding leads to the biologically active proteins.

【 授权许可】

Unknown