Effects of Prunus cerasus L. Seeds and Juice on Liver Steatosis in an Animal Model of Diet-Induced Obesity

Nutrients

Michele Moruzzi¹  Federica Maggi²  Carlotta Marini³  Consuelo Amantini³  Andrea Marchegiani³  MariaGabriella Gabrielli³  Fabrizio Dini³  Alessandro Fruganti³  Daniele Tomassoni³  Giulio Lupidi⁴  Carlo Polidori⁴  Francesco Amenta⁴  MariaVittoria Micioni Di Bonaventura⁴  Carlo Cifani⁴  SeyedKhosrow Tayebati⁴  Ilenia Martinelli⁴ 

[1] Department of Medicine, University of Leipzig, 04103 Leipzig, Germany;Department of Molecular Medicine, University “Sapienza”, 00185 Rome, Italy;School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy;School of Pharmacy, University of Camerino, 62032 Camerino, Italy;

关键词: diet-induced obese rats;    liver;    obesity;    tart cherry;    anthocyanins;   

DOI  :  10.3390/nu12051308

来源: DOAJ

【 摘 要 】

The accumulation of adipose tissue increases the risk of several diseases. The fruits-intake, containing phytochemicals, is inversely correlated with their development. This study evaluated the effects of anthocyanin-rich tart cherries in diet-induced obese (DIO) rats. DIO rats were exposed to a high-fat diet with the supplementation of tart cherry seeds powder (DS) and seed powder plus juice (DJS). After 17 weeks, the DIO rats showed an increase of body weight, glycaemia, insulin, and systolic blood pressure. In the DS and DJS groups, there was a decrease of systolic blood pressure, glycaemia, triglycerides, and thiobarbituric reactive substances in the serum. In the DJS rats, computed tomography revealed a decrease in the spleen-to-liver attenuation ratio. Indeed, sections of the DIO rats presented hepatic injury characterized by steatosis, which was lower in the supplemented groups. In the liver of the DIO compared with rats fed with a standard diet (CHOW), a down-regulation of the GRP94 protein expression and a reduction of LC3- II/LC3-I ratio were found, indicating endoplasmic reticulum stress and impaired autophagy flux. Interestingly, tart cherry supplementation enhanced both unfolded protein response (UPR) and autophagy. This study suggests that tart cherry supplementation, although it did not reduce body weight in the DIO rats, prevented its related risk factors and liver steatosis.

【 授权许可】

Unknown