Food and Agricultural Immunology | |
Development and validation of an immunochromatography test strip for rapid detection of pyrimethanil residues | |
Qiming Kou1  Qi Sun1  Tao Le1  Yarong Wang1  Yi Cao2  Yong Xie2  | |
[1] College of Life Science, Chongqing Normal University;Department of Environmental Art and Design, Chongqing College of Electronic Engineering; | |
关键词: pyrimethanil; immunochromatography test strip; colloidal gold; fungicide residues; food analysis; | |
DOI : 10.1080/09540105.2020.1733496 | |
来源: DOAJ |
【 摘 要 】
Pyrimethanil is one of anilinopyrimidine fungicides widely used in crop protection and frequently found in a variety of crops, including fruits, vegetables, and cereals. This paper describes the development of the first reported immunochromatography test strip (ICTS) based on colloidal gold-labeled monoclonal antibody for the detection of pyrimethanil. Under optimal conditions, the visual cut-off value of ICTS for pyrimethanil was 40 ng/mL. Using a portable strip reader, the 50% inhibition concentration and calculated LOD of pyrimethanil in the PBS samples were 4.8 and 0.9 ng/mL, respectively. When used to analyse fortified samples of fruit and vegetable, acceptable recovery rates of 83.3–105.7% with the coefficient of variation values below 11.7% were obtained. Analysis of blindly fortified samples by the proposed ICTS gave similar results to those of the ic-ELISA and HPLC-MS/MS methods. These results indicate the proposed ICTS is a rapid, simple analytical method for quantitative on-site monitoring of pyrimethanil in foodstuffs.
【 授权许可】
Unknown