High Predatory Capacity of a Novel Arthrobotrys oligospora Variety on the Ovine Gastrointestinal Nematode Haemonchus contortus (Rhabditomorpha: Trichostrongylidae)

Pathogens

Gabriela Aguilar-Tipacamú¹  Cristiana Cazapal-Monteiro²  Maria Sol Arias-Vázquez²  Roberto González-Garduño³  Juan Mosqueda⁴  Fabián Arroyo-Balán⁴  Edgardo Ulises Esquivel-Naranjo⁵  Fidel Landeros-Jaime⁵ 

[1]C.A. Salud Animal y Microbiologia Ambiental, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av. de las Ciencias s/n Col Juriquilla, Querétaro 76230, Mexico

[2]COPAR (Control of Parasites), Animal Pathology Department, Veterinary Faculty, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain

[3]Centro Regional Universitario Sursureste, Universidad Autónoma Chapingo, Teapa 86800, Mexico

[4]Immunology and Vaccines Laboratory, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro 76140, Mexico

[5]Laboratorio de Microbiología Molecular, Unidad de Microbiología Básica y Aplicada, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro 76140, Mexico

关键词: parasitosis;    antihelmintic;    biocontrol;    nematofagous fungi;    Arthrobotrys oligospora;    light response;   

DOI  :  10.3390/pathogens10070815

来源: DOAJ

【 摘 要 】

With the worldwide development of anthelmintic resistance, new alternative approaches for controlling gastrointestinal nematodes in sheep are urgently required. In this work, we identified and characterized native nematode-trapping fungi. We collected seven isolates of fungi with the capacity to form adhesive, three-dimensional networks as the main mechanism to capture, kill, and consume nematodes. The nematode-trapping fungi were classified into two groups; the first group includes the R2-13 strain, showing faster growth, abundant aerial hyphae, scarce conidia production, bigger conidia, and it formed a clade with Arthrobotrys oligospora sensu stricto. The second comprises the A6, A12, A13, R2-1, R2-6, and R2-14 strains, showing a growth adhering to the culture medium, forming little aerial hyphae, smaller conidia, and these formed a sister clade to A. oligospora. Except for the R2-6 strain, conidia production was induced by light. In all the strains, the predatory capacity against the sheep gastrointestinal nematode Haemonchus contortus was greater than 58% compared with the control group. The A6 and A13 strains were the most active against the infective H. contortus third instar (L₃) larvae, with an average capture capacity of 91%. Altogether, our results support evidence for a novel A. oligospora variety with high nematode-trapping activity and promissory in helminthic control.

【 授权许可】

Unknown