Comparison of genomes of African swine fever virus isolates from Cameroon, other African countries and Europe

Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux

N. F. Ekue¹  P. J. Wilkinson² 

[1] IRAD Nkolbisson, BP 2067, Yaounde, Cameroon;The Institute of Animal Health, Pirbright Laboratory, Pirbright, Surrey, GU24 ONF, UK;

关键词: Porcin;    Virus peste porcine africaine;    Génome;    Cameroun;    Europe;   

DOI  :  10.19182/remvt.9717

来源: DOAJ

【 摘 要 】

A comparison of genomes of African swine fever virus (ASFV) isolates from Cameroon, other African countries and Europe by restriction enzyme analysis showed that those from Cameroon and Europe were very closely related except for the Lisbon/57 genome which had a longer left terminal region. Also, the genomes of isolates from Angola, Democratic Republic of Congo (DRC) and Senegal were similar to those from Cameroon and Europe, although the isolates from Angola and Katanga, in DRC, had a long left terminal region similar in size to the Lisbon/57. Isolates from East and Southeast Africa were genetically different from Cameroon and European isolates and also different from each other. Comparison of BamHI restriction enzyme site maps of the virus genomes of Cameroon and European isolates showed a variation in size of the BamHI-L fragment which maps to the middle of the genome, while a comparison between Cameroon, West and Southwest African isolates showed that there were variations in the central and both termini of the genome. Although small differences were observed between genomes of ASF virus isolates from Cameroon, Europe, West and Southwest Africa, they could be considered to belong to the same group based on the similar restriction enzyme site maps of their genomes. The results of this study also showed that the persistent outbreaks of ASF in domestic pig populations in Cameroon might have been due to the re-introduction of closely related virus isolates from infected areas outside the country.

【 授权许可】

Unknown