Changes of the Gastric Mucosal Microbiome Associated With Histological Stages of Gastric Carcinogenesis

Frontiers in Microbiology

Wenming Wu¹  Ranran Zeng¹  Zikai Wang¹  Yunsheng Yang¹  Bin Yan¹  Lili Wu¹  Xiaomei Zhang¹  Rongrong Ren¹  Lihua Peng¹  Qiong Wu¹  Mingzhou Guo¹  Gang Sun¹  Xuefeng Gao²  Huaibo Sun³ 

[1] Department of Gastroenterology and Hepatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China;Department of Hematology-Oncology, International Cancer Center, Shenzhen University General Hospital, Shenzhen University Health Science Center, Shenzhen, China;Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China;

关键词: gastric microbiota;    gastric cancer;    intraepithelial neoplasia;    intestinal metaplasia;    chronic gastritis;   

DOI  :  10.3389/fmicb.2020.00997

来源: DOAJ

【 摘 要 】

The changes of gastric microbiome across stages of neoplastic progression remain poorly understood, especially for intraepithelial neoplasia (IN) which has been recognized as a phenotypic bridge between atrophic/intestinal metaplastic lesions and invasive cancer. The gastric microbiota was investigated in 30 healthy controls (HC), 21 non-atrophic chronic gastritis (CG), 27 gastric intestinal metaplasia (IM), 25 IN, and 29 gastric cancer (GC) patients by 16S rRNA gene profiling. The bacterial diversity, and abundances of phyla Armatimonadetes, Chloroflexi, Elusimicrobia, Nitrospirae, Planctomycetes, Verrucomicrobia, and WS3 reduced progressively from CG, through IM, IN to GC. Actinobacteria, Bacteriodes, Firmicutes, Fusobacteria, SR1, and TM7 were enriched in the IN and GC. At the community level, the proportions of Gram-positive and anaerobic bacteria increased in the IN and GC compared to other histological types, whereas the aerobic and facultatively anaerobic bacteria taxa were significantly reduced in GC. Remarkable changes in the gastric microbiota functions were detected after the formation of IN. The reduced nitrite-oxidizing phylum Nitrospirae together with a decreased nitrate/nitrite reductase functions indicated nitrate accumulation during neoplastic progression. We constructed a random forest model, which had a very high accuracy (AUC > 0.95) in predicating the histological types with as low as five gastric bacterial taxa. In summary, the changing patterns of the gastric microbiota composition and function are highly indicative of stages of neoplastic progression.

【 授权许可】

Unknown