Usage of chi-Sequence for Differentiation between the Strains of Anthrax Etiological Agent and Closely Related Bacilli

Проблемы особо опасных инфекций

A. N. Mokrievich¹  L. I. Marinin¹  T. Yu. Kudryavtseva¹  G. M. Vakhrameeva¹  I. A. Dyatlov¹  N. A. Shishkova¹  V. M. Pavlov¹ 

[1] State Research Center for Applied Microbiology and Biotechnology;

关键词: bacillus anthracis;    дифференциация;    пцр;    chi-последовательность;    bacillus anthracis;    differentiation;    pcr;    chi-sequence;   

DOI  :  10.21055/0370-1069-2014-2-97-100

来源: DOAJ

【 摘 要 】

Objective of the study was to carry out comparison between the microorganisms appurtenant to six different species of Bacilli genus as regards mobility of the amplicons obtained using the primer that contained chi-sequence of Bacillus subtilis . Therewith, analyzed were 30 Bacillus anthracis strains and closely related bacilli. Cultures grew on L-agar and L-broth at 37 °C. DNA was isolated from vegetative B. anthracis and Bacillus spp . cultures introducing lysozyme and phenol de-proteinizing. In order to perform single-primer PCR constructed was ChiBs primer - 5’-CTAGGAGCGGG-3’. Single-primer amplification (30 cycles) was carried out at annealing temperature of 47 °C. Comparative analysis of individual electrophoretic amplicon profiles, obtained by means of ChiBs-primer PCR, allows for identification of genetic intraspecific variability and differentiation of B. anthracis strains from the atypical ones and closely related species of bacilli.

【 授权许可】

Unknown