Characterization of Developing Cotton Fibers by Confocal Raman Microscopy

Fibers

Luis Cabrales¹  Felicia Manciu²  Noureddine Abidi³ 

[1] Department of Physics and Engineering, California State University at Bakersfield,9001 Stockdale Highway, Bakersfield, CA 93311, USA;Department of Physics, University of Texas at El Paso, El Paso, TX 79968, USA;Fiber and Biopolymer Research Institute, Department of Plant and Soil Science,Texas Tech University, Lubbock, TX 79403, USA;

关键词: cotton;    confocal Raman microscopy;    X-Ray Diffraction (XRD);    cellulose crystallinity;    fiber development;    label-free imaging;   

DOI  :  10.3390/fib2040285

来源: DOAJ

【 摘 要 】

Cellulose deposition in developing cotton fibers has been studied previously with analytical techniques, such as Fourier transform infrared spectroscopy (FTIR),High-performance liquid chromatography (HPLC) and Thermogravimetric analysis (TGA). Recent technological developments in instrumentation have made Raman microscopy emerge as an extraordinary analytical tool in biological and plant research. The advantage of using confocal Raman microscopy (CRM) resides in the lateral spatial resolution and in the fact that Raman spectroscopy provides not only chemical composition information, but also structural information. Cross-sections of cotton fibers harvested at different developmental stages were studied with CRM. The Raman bands assigned to cellulose were analyzed. The results of this study indicate that CRM can be used as a tool to study cellulose deposition in cotton fibers and could provide useful information on cellulose deposition during cotton fiber development.

【 授权许可】

Unknown