An Integrated Process of Ionic Liquid Pretreatment and Enzymatic Hydrolysis of Lignocellulosic Biomass with Immobilised Cellulase

BioResources

Mihaela Ungurean¹  Francisc Péter¹  Zsófia Csanádi²  László Gubicza² 

[1] University Politehnica of Timisoara;University of Pannonia;

关键词: Pretreatment;    Ionic liquid;    Poplar biomass;    Cellulose hydrolysis;    Immobilised cellulase;   

DOI  :  10.15376/biores.9.4.6100-6116

来源: DOAJ

【 摘 要 】

An integrated process of lignocellulosic biomass conversion was set up involving pretreatment by an ionic liquid (IL) and hydrolysis of cellulose using cellulase immobilised by the sol-gel method, with recovery and reuse of both the IL and biocatalyst. As all investigated ILs, regardless of the nature of the anion and the cation, led to the loss of at least 50% of the hydrolytic activity of cellulase, the preferred solution involved reprecipitation of cellulose and lignin after the pretreatment, instead of performing the enzymatic hydrolysis in the same reaction system. The cellulose recovered after pretreatment with 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]) and dimethylsulfoxide (DMSO) (1:1 ratio, v/v) was hydrolysed with almost double yield after 8 h of reaction time with the immobilised cellulase, compared to the reference microcrystalline cellulose. The dissolution capacity of the pretreatment mixture was maintained at satisfactory level during five reuse cycles. The immobilised cellulase was recycled in nine reaction cycles, preserving about 30% of the initial activity.

【 授权许可】

Unknown