Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage

Neural Regeneration Research

关键词: tamoxifen;    Src kinase;    PP2;    trauma;    regeneration;    neuroprotection;    auranofin;    dextromethorphan;    rosiglitazone;    Alzheimer′s disease;    neuroinflammation;    neurodegeneration;    microglia;    astrocytes;    nerve regeneration;    spinal cord;    electroacupuncture therapy;    neural stem cells;    notch signaling pathway;    astrocytes;    inflammation;    survival curve;    proliferation;    differentiation;    real-time quantitative PCR;    western blot assay;    neural regeneration;    nerve regeneration;    superparamagnetic iron oxide;    magnetic guidance;    bone marrow mesenchymal stem cells;    spinal cord injury;    cell transplantation;    magnetic resonance image;    lumbar puncture;    neural regeneration;    nerve regeneration;    spinal cord injury;    spinal cord transection;    average combined score;    magnetic resonance imaging;    diffusion tensor imaging;    fractional anisotropy;    apparent diffusion coefficient;    fiber tractography;    neural regeneration;    nerve regeneration;    peripheral nerve injury;    sciatic nerve;    hypothermia;    blood-nerve barrier;    Evans blue tracer;    neural degeneration;    nerve regeneration;    polyethyleneimine-polyethylene glycol;    spiral ganglion cells;    X-linked inhibitor of apoptosis protein;    gene therapy;    nanocarrier;    cisplatin;    neural regeneration;    ototoxicity;    cochlea;   

DOI  :  10.4103/1673-5374.153691

来源: DOAJ

【 摘 要 】

Polyethyleneimine-polyethylene glycol (PEI-PEG), a novel nanocarrier, has been used for transfection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein (XIAP) in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Auditory brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.

【 授权许可】

Unknown