Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots

Molecules

Pablo Morales-Tapia¹  Ady Giordano²  Mauricio Moncada-Basualto³  Josué Pozo-Martínez³  Claudio Olea-Azar³  Aleksandra Nesic⁴  Gustavo Cabrera-Barjas⁴ 

[1] Escuela de Ciencias Agrícolas y Veterinarias, Universidad Viña del Mar, Agua Santa 7055, Viña del Mar 2531015, Chile;Inorganic Chemistry Department, Faculty of Chemistry and Pharmacy, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Santiago 8330077, Chile;Laboratory of Free Radicals and Antioxidants, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Sergio Livingstone Polhammer 1007, Independencia 7820436, Chile;Unidad de Desarrollo Tecnológico (UDT), Universidad de Concepción, Avenida Cordillera 2634, Parque Industrial Coronel, Concepción 3349001, Chile;

关键词: Argylia radiata;    vitroplants;    polyphenolic extracts;    antioxidant activity;    ORAC;    electronic paramagnetic resonance (EPR);   

DOI  :  10.3390/molecules27030610

来源: DOAJ

【 摘 要 】

Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.

【 授权许可】

Unknown