Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis

Marine Drugs

JoséA. Pérez¹  Ana Galindo¹  Covadonga Rodríguez¹  Manuel Marrero¹  Diego Garrido¹  Inmaculada Giráldez²  Marcelino Herrera³  Mónica Betancor⁴  Óscar Monroig⁵ 

[1] Departamento de Biología Animal, Edafología y Geología, Universidad de La Laguna, 38200 San Cristóbal de La Laguna, Spain;Departamento de Química “Profesor Carlos Vílchez Martín”, Universidad de Huelva, 21007 Huelva, Spain;IFAPA Centro Agua del Pino, 21450 Cartaya, Spain;Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK;Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC), 12595 Ribera de Cabanes, Spain;

关键词: Solea senegalensis;    Fads2;    Elovl5;    LC-PUFA biosynthesis;    diet;    salinity;   

DOI  :  10.3390/md19050254

来源: DOAJ

【 摘 要 】

Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in Solea senegalensis the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-¹⁴C] 18:3 n-3, and the regulation of the S. senegalensis fads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing S. senegalensis, has at least one Fads2 with ∆6 activity. Dietary composition prevailed over salinity in regulating the expression of fads2, while salinity did so over dietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensis muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.

【 授权许可】

Unknown