A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films

Nanomaterials

Zidong Li¹  AlexanderE. Hampe¹  Erkin Seker²  Sunjay Sethi³  PamelaJ. Lein³ 

[1] Department of Biomedical Engineering, University of California—Davis, Davis, CA 95616, USA;Department of Electrical and Computer Engineering, University of California—Davis, Davis, CA 95616, USA;Department of Molecular Biosciences, University of California—Davis, Davis, CA 95616, USA;

关键词: nanostructure;    cell-material interaction;    nanoporous gold;    adhesion strength;    astrocyte;    focal adhesion;    microfluidic flow-cell;   

DOI  :  10.3390/nano8070452

来源: DOAJ

【 摘 要 】

Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed.

【 授权许可】

Unknown