Label-Free Cholesteric Liquid Crystal Biosensing Chips for Heme Oxygenase-1 Detection within Cerebrospinal Fluid as an Effective Outcome Indicator for Spontaneous Subarachnoid Hemorrhage

Biosensors

Hui-Tzung Luh¹  Yu-Cheng Hsiao²  Po-Yi Cho²  Yi-Wei Chung³ 

[1] Department of Neurosurgery, Taipei Medical University-Shuang Ho Hospital, New Taipei 23561, Taiwan;Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan;Graduate Institute of Clinical Medicine, National Taiwan University, Taipei 10617, Taiwan;

关键词: cholesteric liquid crystal;    heme oxygenase-1;    cerebrospinal fluid;    spontaneous subarachnoid hemorrhage;   

DOI  :  10.3390/bios12040204

来源: DOAJ

【 摘 要 】

A novel device for cholesteric liquid crystal (LC; CLC)-based biosensing chips for detecting heme oxygenase (HO)-1 within the cerebrospinal fluid (CSF) was invented. In the CLC device, the reorientation of the LCs was strongly influenced by the alignment layer surface and adjacent LCs. When the substrate was coated with the alignment layer, the CLCs oriented homeotropically in a focal conic state. Once HO-1 was immobilized onto the orientation sheet-coated substrate, the CLC changed from a focal conic state to a bright planar state by disrupting the CLCs. The concentration of HO-1 within CSF was shown to be an effective outcome indicator for patients with a spontaneous subarachnoid hemorrhage. We showed that the CLC immunoassaying can be used to measure HO-1 with a lower detection limit of about 10 ng/mL. The linear range was 10 ng/mL to 1 mg/mL. An easy-to-use, rapid-detection, and label-free CLC immunoassay device is proposed.

【 授权许可】

Unknown