Observation Interface of PDMS Membrane in a Microfluidic Chip Based on One-Step Molding

Micromachines

Xiangyu Chen¹  Penghui Xiong¹  Jian Chu²  Shuangyue Hou³  Gang Liu³  Yangchao Tian³  Ying Xiong³ 

[1] Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China;Institute of Materials, China Academy of Engineering Physics, Mianyang 621700, China;National Synchrotron Radiation Laboratory, Unitersity of Science and Technology of China, Hefei 230029, China;

关键词: microfluidic chip;    polydimethylsiloxane (PDMS);    PDMS membrane;    imaging;   

DOI  :  10.3390/mi8030064

来源: DOAJ

【 摘 要 】

Nowadays, researchers are focusing on sorting, characterizing and detecting micron or submicron particles or bacteria in microfluidic chips. However, some contradictions hinder the applications of conventional microfluidic chips, including the low working distance of high resolving power microscopy and the low light transmittance of conventional microfluidic chips. In this paper, a rapid and readily accessible microfluidic fabrication method is presented to realize observation with high magnification microscopy. With the one-step molding process, the interconnections, the thin observation interface of polydimethylsiloxane (PDMS) membrane and microfluidic channels were integrated into an intact PDMS replica. Three kinds of PDMS replicas with different auxiliary beams were designed and optimized by leakage experiments and analytical software. The observation interfaces of a 170 μm thickness PDMS membrane enlarges the application domain of microfluidic chips. By adopting a solution of high magnification observation, microfluidic devices could be applied widely in medical science, biology and material science.

【 授权许可】

Unknown