A Glossary for Chemical Approaches towards Unlocking the Trove of Metabolic Treasures in Actinomycetes

Molecules

Eman Maher Zahran¹  Usama Ramadan Abdelmohsen²  Heba Ali Hassan³  Jianye Zhang⁴ 

[1] Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City 61111, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt;Key Laboratory of Molecular Target & Clinical Pharmacology and the State & NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China;

关键词: Actinomycetes;    antibiotics biosynthesis;    cryptic genes;    elicitors;    biological potential;   

DOI  :  10.3390/molecules27010142

来源: DOAJ

【 摘 要 】

Actinobacterial natural products showed a critical basis for the discovery of new antibiotics as well as other lead secondary metabolites. Varied environmental and physiological signals touch the antibiotic machinery that faced a serious decline in the last decades. The reason was exposed by genomic sequencing data, which revealed that Actinomycetes harbor a large portion of silent biosynthetic gene clusters in their genomes that encrypt for secondary metabolites. These gene clusters are linked with a great reservoir of yet unknown molecules, and arranging them is considered a major challenge for biotechnology approaches. In the present paper, we discuss the recent strategies that have been taken to augment the yield of secondary metabolites via awakening these cryptic genes in Actinomycetes with emphasis on chemical signaling molecules used to induce the antibiotics biosynthesis. The rationale, types, applications and mechanisms are discussed in detail, to reveal the productive path for the unearthing of new metabolites, covering the literature until the end of 2020.

【 授权许可】

Unknown