Modeling of Zymomonas mobilis central metabolism for novel metabolic engineering strategies

Frontiers in Microbiology

Uldis eKalnenieks¹  Reinis eRutkis¹  Agris ePentjuss²  David A Fell³  Egils eStalidzans⁴ 

[1] Institute of Microbiology and Biotechnology, University of Latvia;Latvia University of Agriculture;Oxford Brookes University;SIA TIBIT;

关键词: Metabolic Engineering;    Systems Biology;    kinetic modeling;    central metabolism;    stoichiometric modeling;    elementary flux modes;   

DOI  :  10.3389/fmicb.2014.00042

来源: DOAJ

【 摘 要 】

Mathematical modeling of metabolism is essential for rational metabolic engineering. The present work focuses on several types of modeling approach to quantitative understanding of central metabolic network and energetics in the bioethanol-producing bacterium Zymomonas mobilis. Combined use of Flux Balance, Elementary Flux Mode and thermodynamic analysis of its central metabolism, together with dynamic modeling of the core catabolic pathways, can help to design novel substrate and product pathways by systematically analysing the solution space for metabolic engineering, and yields insights into the function of metabolic network, hardly achievable without applying modeling tools.

【 授权许可】

Unknown