An Electrochemical Impedimetric Aptasensing Platformfor Sensitive and Selective Detection of Small MoleculesSuch as Chloramphenicol

Sensors

Tom Breugelmans¹  Tarryn Dierckx¹  Sanaz Pilehvar²  Karolien De Wael²  Ronny Blust³ 

[1] ART Research Group, Faculty of Applied Engineering, University of Antwerp, Salesianenlaan 90, 2000 Antwerp, Belgium;AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium;Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium;

关键词: aptasensor;    electrochemical impedance spectroscopy;    chloramphenicol;    specific recognition;   

DOI  :  10.3390/s140712059

来源: DOAJ

【 摘 要 】

We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of theaptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.

【 授权许可】

Unknown