EMC1-dependent stabilization drives membrane penetration of a partially destabilized non-enveloped virus

eLife

Billy Tsai¹  Takamasa Inoue¹  Parikshit Bagchi¹ 

[1] Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, United States;

关键词: protein stabilization;    membrane transport;    viral entry;   

DOI  :  10.7554/eLife.21470

来源: DOAJ

【 摘 要 】

Destabilization of a non-enveloped virus generates a membrane transport-competent viral particle. Here we probe polyomavirus SV40 endoplasmic reticulum (ER)-to-cytosol membrane transport, a decisive infection step where destabilization initiates this non-enveloped virus for membrane penetration. We find that a member of the ER membrane protein complex (EMC) called EMC1 promotes SV40 ER membrane transport and infection. Surprisingly, EMC1 does so by using its predicted transmembrane residue D961 to bind to and stabilize the membrane-embedded partially destabilized SV40, thereby preventing premature viral disassembly. EMC1-dependent stabilization enables SV40 to engage a cytosolic extraction complex that ejects the virus into the cytosol. Thus EMC1 acts as a molecular chaperone, bracing the destabilized SV40 in a transport-competent state. Our findings reveal the novel principle that coordinated destabilization-stabilization drives membrane transport of a non-enveloped virus.

【 授权许可】

Unknown