Prevention of di (2-ethylhexyl) Phthalate-induced Testicular Disturbance in Mice by Co-administration of L-carnitine

International Journal of Fertility and Sterility

Majid Malekzadeh Shafaroudi¹  Hossein Eimani²  Zohre Zare³  Moslem Mohammadi⁴ 

[1] 4Department of Anatomy, Mazandaran University of Medical Sciences, Sari, Iran;Department of Anatomy, Baqiatallah University of Medical Sciences, Tehran, Iran;Department of Anatomy, Shahid Beheshti University of Medical Sciences, Tehran, Iran;Department of Physiology and Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran;

关键词: l;    carnitine;    di (2;    ethylhexyl) phthalate;    spermatogenesis;    testis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Backgrounddi (2-ethylhexyl) phthalate (DEHP) is widely used in the plastic industry and caninduce reproductive toxicity. On the other hand, L-carnitine (LC) plays a crucial role in spermmetabolism and maturation. This study evaluates the effect of LC on body and testis weight,testis tissue, count, motility, viability, morphology, and chromatin quality of epididymal sperm,testicular spermatid number (TSN) per gram testis and daily sperm production (DSP) in LC-treated mice.Materials and MethodsIn this experimental study, adult male NMRI mice (mean age: 4weeks) were given doses of DEHP and LC by gavaging for 2 weeks. All samples were assessedaccording to World Health Organization (WHO) criteria. Sperm morphology was assessed usingPapanicolaou staining and sperm chromatin quality by aniline-blue staining.The left testes were fixed in Bouinś solution for histological examination and the end slices werestained with hematoxylin and eosin (H&E). The right testes were homogenized, and then TSNand DSP were calculated with an improved Neubauer haemocytometer and respective frames.Paired t-test, ANOVA, and Kruskal-Wallis tests were utilized for data analysis.ResultsCo-administration of DEHP and LC not only prevented significant gains in testicularweight, but also maintained the sperm’s normal morphology and chromatin quality (p < 0.05). Inaddition, LC recovered histological changes, TSN, DSP, and sperm count.ConclusionThese results demonstrated that oral administration of LC partially or generallyprotects spermatogenesis from DEHP-toxicity in mice.

【 授权许可】

Unknown