Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development

eLife

Juan-Pablo Labrador¹  Jost Vielmetter²  Agnieszka Olechwier²  Michael Anaya³  Kai Zinn⁴  K Christopher Garcia⁵  Mario A Fares⁵  Hanqing Li⁵  Siamak K Sorooshyari⁵  Dermott P Harnett⁶  Hyung-Kook (Peter) Lee⁷  Engin Özkan⁷  Ash Watson⁷ 

[1]Department of Abiotic Stress, Group of Integrative and Systems Biology, Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Valencia, Spain

[2]Institute of Neuroscience, Trinity College Dublin, University of Dublin, Dublin, Ireland

[3]Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, United States

[4]Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, United States

[5]Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States

[6]Ellipsis Health, San Francisco, United States

[7]Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland

关键词: protein-protein interaction;    interactome;    immunglobulin superfamily;    neural development;    motor neuron;    motor axon guidance;   

DOI  :  10.7554/eLife.28111

来源: DOAJ

【 摘 要 】

An ‘interactome’ screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we ‘deorphanized’ four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.

【 授权许可】

Unknown