Rice Sesquiterpene Plays Important Roles in Antixenosis against Brown Planthopper in Rice

Plants

Apichart Vanavichit¹  Sugunya Mahatheeranont²  Pornwalai Thorngkham²  Kitsada Pitija²  Wissarut Sukhaket³  Theerayut Toojinda⁴  Wintai Kamolsukyeunyong⁴ 

[1] Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom 73140, Thailand;Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;Expert Center of Innovative Agriculture, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Technopolis, Khlong Ha, Khlong Luang, Pathum Thani 12120, Thailand;National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, Thailand;

关键词: brown planthopper;    terpene synthase;    E-β-farnesene;    transcription factor binding site;   

DOI  :  10.3390/plants10061049

来源: DOAJ

【 摘 要 】

The rice sesquiterpene synthase II gene (OsSTPS2, LOC_Os04g27430), which is involved in the antixenosis defense mechanism of rice against brown planthopper (BPH) infestation, was identified in the BPH-resistant rice variety Rathu Heenati (RH). In contrast, the gene was not functional in the BPH-susceptible rice variety KDML105 (KD). Single-nucleotide polymorphisms (SNPs) in the promoter region and in exon 5 of the gene and a seven amino acid deletion in the deduced protein sequence are suggested as factors that negatively regulate the function of the gene. Sequence analysis of the promoter region and expression analysis of the OsSTPS2 gene in several rice genotypes revealed the correlation of SNPs of the ATHB-1, SBE1, and P-factor with the expression of the gene. Genomic and complementary DNA (cDNA) sequence analysis at exon 5 of the gene showed that the 21 bp deletion naturally occurred in several rice genotypes. The antixenosis of the BPH feeding preference (AFP) of rice varieties differed in the seven amino acid deletion lesion of the gene, suggesting that the seven amino acid deletion negatively controls the antixenosis mechanism during BPH infestation. Analysis of the plant volatile compounds released after BPH infestation suggested that E-β-farnesene (EBF) is the major product of the OsSTPS2 gene.

【 授权许可】

Unknown